Folate (folic acid): what it is, what it tells you and when it’s tested
Folate is a B vitamin, B9. You find it in leafy greens, legumes and citrus fruit. Folic acid is the form used in supplements. Your body needs it to make red blood cells and build new cells. The test measures how much folate is in your blood.
When it’s tested
Doctors usually order folate together with vitamin B12. They do so when a blood count shows anaemia with large red cells, if you eat few vegetables, drink a lot of alcohol, have a condition that reduces absorption, such as coeliac disease, or take medicines that lower it.
It is not a routine test for people who feel well. Blood folate changes within days with what you eat, so a single result says little about your store. And a shortage that matters usually already shows on a blood count.
That is why Rosa does not test it. Instead it looks at vitamin B12, MCV and haemoglobin, which show whether a B12 or folate shortage is already changing your red blood cells.
What it tells you, and what it does not
If folate is low, you have taken in little over the last days or weeks, or your body is using or losing more.
On its own it does not say whether you have anaemia, or whether B12 is short too. A blood count and B12 answer that.
If it is normal, your folate is usually enough. If it is high, you almost always take a supplement.
That is why it is read together with other tests:
- Vitamin B12: The two shortages look alike and often go together
- MCV: Large red cells point to B12 or folate
- Haemoglobin: It shows whether the shortage has caused anaemia
- Homocysteine: It rises when folate runs short
Normal folate levels
The lab decides what is normal, and it depends on the method. Many lab reports give 3.9 to 26.8 ng/mL. Some labs write µg/L, which is the same number, others nmol/L, with larger numbers.
Below 3 ng/mL there is usually a real shortage. Between 3 and your report’s limit there is a grey zone, to be read with a blood count and B12.
There is also a red cell folate test. It reflects the previous months, not just recent days, but it is rarely used.
What your result means
Find the row for your result, in ng/mL. Which values need a doctor? Those below your report’s limit, especially below 3.
- Below 3: Very low. Usually a real folate shortage. Below this level the risk of anaemia rises sharply. Talk to your doctor: they usually check B12 and a full blood count before any treatment.
- 3 to 3.9: Low. Below the limits on many lab reports, but in a grey zone. It often reflects little folate in recent days. Show it to your doctor, with B12 and a blood count. Often a change in what you eat is enough.
- 3.9 to 26.8: Normal. You are getting enough folate, at least in the days before the test. Nothing to do.
- Above 26.8: High. Almost always it means you take folic acid or a multivitamin. On its own it is not a problem. Tell your doctor. If you take folic acid, ask for your B12 to be checked too.
If your folate is low or high
- Low folate: Usually too little folate in your diet, alcohol, coeliac disease or some medicines. It often corrects well.
- High folate: Almost always a supplement. Not a problem in itself, but B12 should be checked.
Folic acid before pregnancy
This is where folic acid matters most. If you are planning a pregnancy, or do not rule one out, the Italian recommendation is 0.4 mg of folic acid a day, from at least one month before conception until the third month of pregnancy.
It lowers the risk of some birth defects, such as spina bifida. You do not need a blood test to start: it applies to every woman in that situation.
In some cases the doctor advises a different dose. Talk to your doctor or gynaecologist.
What can skew the result
Blood folate follows what you have eaten recently. It helps to know this before your blood test.
- A recent meal or supplement: Either can raise it for a few hours. That is why the test is usually done fasting.
- A few days of poor diet: They can lower it even if your store is still fine. One result is not enough.
- Alcohol: People who drink a lot often have low folate, because they absorb and keep less of it.
- Some medicines: Methotrexate, some anti-epileptics and some antibiotics can lower it. Do not stop them on your own: talk to your doctor.
When to worry
Talk to your doctor if:
- Your folate is below your report’s limit, especially below 3 ng/mL.
- It is low and B12 is low too, or your MCV is high.
- You have tiredness that does not go away, shortness of breath, a red tongue or frequent mouth ulcers.
- You are planning a pregnancy: talk to your doctor or gynaecologist about folic acid.
- You have chest pain or find it very hard to breathe: call 112 or go to the emergency department.
Frequently asked questions
What is the difference between folate and folic acid? They are the same vitamin, B9. Folate is the name for the forms in food. Folic acid is the form used in supplements. On an Italian lab report the test may be called folati, folato sierico or acido folico.
What are normal folate levels? Many lab reports give 3.9 to 26.8 ng/mL, but it depends on the lab’s method. The range printed next to your result is what counts.
Do I need to fast for a folate test? Usually yes, because a recent meal or supplement raises it. You can drink water.
Should I have a folate test before pregnancy? Usually not. Folic acid is advised for every woman planning a pregnancy, without a test. A doctor orders it only in particular cases, for example if there is anaemia.
Does Rosa test folate? No. Rosa measures vitamin B12, MCV and haemoglobin, which show whether a B12 or folate shortage is changing your red blood cells. If your doctor wants folate, they order it separately.
Sources
- Devalia and colleagues, guidelines for the diagnosis and treatment of cobalamin and folate disorders. British Journal of Haematology, 2014. PMID 24942828
- Cordero and colleagues, optimal serum and red blood cell folate concentrations in women of reproductive age for prevention of neural tube defects: World Health Organization guidelines. MMWR, 2015. PMID 25905896
- Istituto Superiore di Sanità, EpiCentro: folic acid and folate, the recommendation of the Italian folic acid promotion network. ISS, Network italiano promozione acido folico
- Liverpool University Hospitals, serum folate laboratory test sheet: reference interval of the method in use. Liverpool University Hospitals NHS, serum folate